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HPLC

High-performance liquid chromatography (HPLC is formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. HPLC is used in many different ways. Manufacturers use it during the production process of pharmaceutical and biological products. It can be used for research when separating the components of a complex biological sample or of similar chemicals from each other. It can also be useful in medical and legal purposes.

What is Chromatography

Chromatography can be described as a mass transfer process involving adsorption. HPLC is differentiated from traditional (low pressure) Liquid Chromatography because HPLC relies on pumps and pressurization of liquids, whereas ordinary Liquid Chromatography relies on the force of gravity to pass liquids.

HPLC Laboratory Products

HiTechTrader offers not only parts and accessories (like autosamplers, columns, column heaters/chillers, controllers, data servers, degassers, detectors, fraction collectors, prep, prep systems, pumps, scintillation, solvent recyclers, and valves), but we also offer complete HPLC systems for sale.

HPLC Manufacturers

The most requested manufacturers are Agilent Technologies Inc., Beckman, and Waters Corporation.

We Enjoy Technical Questions

We have been helping customers like you decide between different products since 1982 so send us an inquiry or call us today.

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5433 - Sepragen Corp. Superflo 5oo Column
Number of Items: 79

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002191025

Rheodyne 7725i Sample Injector

    Price: $450.00    
Rheodyne 7725i Sample Injector

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003190928

Sepragen Radial Flow Chromatography Column Superflo 50

    Price: $450.00    
Sepragen Radial Flow Chromatography Column  Superflo 50,  Stainless Steel

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002190928

Sepragen Radial Flow Chromatography Column Superflo 50

    Price: $350.00    
Sepragen Radial Flow Chromatography Column Superflo 50 with column stand and smaller Superflo column

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007190622

Pickering Laboratories PCX Post Column Derivatizer

    Price: $6,500.00    
Pickering Laboratories PCX Post Column Derivatizer  HPLC post-column derivatization system for analysis of Amino Acids, Carbamates, Mycotoxins, Antibiotics and many other applications. ... More Info

  >>     >>  


001171109

Agilent G1376-60002 Flow Sensor 100uL

    Price: $1,100.00    POD Price: $999.00    

Agilent Flow G1376-60002 Sensor For HPLC

Agilent Technologies G1376-6002 part / accessories Flow Sensor for HP Agilent HPLC System 100 ul Max

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001170927

Dionex LC30 Ion Chromatography HPLC Oven

    Price: $750.00    POD Price: $600.00    

Dionex  Thermo Scientific LC30-1 HPLC oven


Thermo Dionex LC30-1 Chromatography HPLC Oven. Operating Temperature: up to 80°C, 1° increments. ... More Info

  >>     >>  


007170926

Dionex AS50 Thermal Compartment

    Price: $299.00    POD Price: $99.00    
Dionex AS50 Thermal Compartment

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004170608

Dionex CD25 Conductivity Detector

    Price: $1,200.00    POD Price: $800.00    
Conductivity Detector CD25 Dionex

Dionex Conductivity Detector CD25. Detects and quantifies ionic analytes in liquid and  ion chromatography. Range;0.01µS to    3000 uS, full scale. ... More Info

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003170608

Dionex GS50 Gradient Pump

    Price: $650.00    POD Price: $650.00    
Dionex Gradient Pump. The GS50 Gradient Pump is a microprocessor-based eluent (or mobile phase) delivery system. It delivers mixtures of up to four mobile phase components at precisely controlled flow rates. Operating temperature: 10 to 50°C (50 to 104°F). Flow accuracy: <1% of set value or ±2 µL/min, whichever is greater. Flow precision: <0.2%. Compositional accuracy: <±1%. Operating pressure: 35 MPa (5000 psi), maximum. ... More Info

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004170601

Dionex AS3500 Spectra System Autosampler

    Price: Please Call    
Dionex Inert Variable Loop Autosampler model AS3500. Dionex AS3500 inert variable loop autosampler with column oven.  ALS provides high-performance automatic sampling at entry level pricing. Configuration flexibility includes fixed volume injection, variable volume injection, column oven, sample preparation, and sample tray temperature control.

... More Info

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003170601

Dionex GS50 Gradient Ion Chromatorgraph Pump

    Price: $999.00    POD Price: $899.00    

Dionex GS50 Gradient Pump for IC HPLC

The GS50 Gradient Pump is a microprocessor-based eluent (or mobile phase) delivery system. It delivers mixtures of up to four mobile phase components at precisely controlled flow rates. Operating temperature: 10 to 50°C (50 to 104°F). Flow accuracy: <1% of set value or ±2 µL/min, whichever is greater. Flow precision: <0.2%. Compositional accuracy: <±1%. Operating pressure: 35 MPa (5000 psi), maximum.
... More Info

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001170601

Dionex LC20 Chromatography Enclosure

    Price: Please Call    
Dionex Chromatography Enclosure model LC2.The LC20 Chromatography Enclosure is an integral part of the Dionex DX 500 Chromatography Systems. It can house the following system components, which must be ordered separately or as part of another module: Separator columns, optional guard columns, SRS (Self-Regenerating Suppressor), DS3 DetectionStabilizer, and detector cell(s) for the ED40 Electrochemical Detector and CD20 Conductivity Detector. The LC20 is available in two configurations, depending on the type of injection valve installed:LC30, PEEK automated injection valve (P/N 044088). LC30, stainless steel automated injection valve (P/N 04417). ... More Info

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007170531

Dionex EG50 Eluent Generator

    Price: Please Call    
Dionex EG50 Eluent Generator. Minimum and Maximum concentration; 0.1-100 mM. Flow rates; 0.10-3.0 mL/min. Max operating pressure; 3000 psi. Operating Temp range; 4-40 C.


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006170531

Dionex ED40 Electrochemical Detector

    Price: Please Call    
Dionex Electrochemical Detector model ED4.The ED40 Electrochemical Detector measures current resulting from the application of potential (voltage) across electrodes in flow-through cells. Depending on the method by which the potential is applied and the current measured, several different properties of theflowing solution can be determined. ... More Info

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001170427

Corona Plus CAD Detector

    Price: $4,300.00    
Corona Plus CAD detector, 70-6967. CoronaPlus detector is designed to be used with mobile phases
that may adversely affect the performance of the detector due to cooling of the
nebulizer (e.g., when using aqueous THF gradients). eliable and simple to operate.The Corona Charged Aerosol Detector offers all-in-one performance benefits that refractive index (RI), low wavelength (UV), evaporative light scattering (ELS), and chemiluminescence nitrogen (CLN) detector methods lack. Its superior performance typically provides ten times the sensitivity of ELS, a broad dynamic detection range over four orders of magnitude, more consistent response factors independent of chemical structure and full gradient capability, Universal detection of nonvolatile and many semi-volatile analytes. Four orders of magnitude dynamic range (nonlinear). Low ng lower limits of detection. Typically < 2% R.S.D. response variability. Response magnitude is minimally dependent on the analyte’s chemical
properties.Broad applicability. Isocratic and gradient compatibility.The
Corona Charged Aerosol Detector offers all-in-one performance benefits
that refractive index (RI), low wavelength (UV), evaporative light
scattering (ELS), and chemiluminescence nitrogen (CLN) detector methods
lack. Its superior performance typically provides ten times the
sensitivity of ELS, a broad dynamic detection range over four orders of
magnitude, more consistent response factors independent of chemical
structure and full gradient capability,
The
Corona Charged Aerosol Detector offers all-in-one performance benefits
that refractive index (RI), low wavelength (UV), evaporative light
scattering (ELS), and chemiluminescence nitrogen (CLN) detector methods
lack. Its superior performance typically provides ten times the
sensitivity of ELS, a broad dynamic detection range over four orders of
magnitude, more consistent response factors independent of chemical
structure and full gradient capability
... More Info

  >>     >>  


003170424

HP Agilent Series 1100 G1316A Column Compartment Oven

    Price: Please Call    
HP/Agilent G1316A Column Compartment /Oven, Series 1100 HPLC.

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001170420

Waters Acquity UPLC System

    Price: $25,000.00    POD Price: $25,000.00    
Waters Acquity Ultra Performance Liquid Chromatography Binary pump and autosampler


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003160713

Hewlett Packard G1314A HPLC 1100 VWD Detector

    Price: $1,499.00    POD Price: $999.00    

Agilent HP G1314A 1100 HPLC Variable Wavelength Detector

Agilent / Hewlett Packard HP HPLC 1100 Model G1314A - Variable Wavelength Detector.  Wavelength Range: 190 - 600 nm, Wavelength Accuracy: +/- 1 nm, Band width: 6.5 nm , Double beam photometer with Deuterium lamp, Linearity: >2 AU (5%) upper limit Drift: 3×10-4 AU/hr at 254 nm, Analog output connection.
... More Info

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001160607

Agilent HPLC ALS Therm G1330B

    Price: $1,345.00    POD Price: $999.00    

Agilent G1330B HPLC Autosampler Heater Chiller

Agilent ALS Therm for  HPLC G1330B
  • ALS Temp Range: -1°C to 4°C
  • ALS Ambient Temp: 4-40°C (41-131°F)
  • ALS Line Voltage: 100-120 or 220-240 VAC +- 10%
... More Info

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004160606

HP Cover Switch Assembly 79846-67903

    Price: $199.00    
Agilent/HP Cover Switch Assembly 79846-67903 for HP 1090 Series L/M Liquid Chromatograph


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001160427

HP Agilent Degasser 1100 Model G1322A

    Price: $1,799.00    
Surplus Hewlett Packard Agilent Series 1100 Model G1322A HPLC Degasser.


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003160217

Agilent Technologies 79846-87604 Sample Loop Capillary

    Price: $50.00    POD Price: $50.00    

Agilent 79846-87604 Loop

Agilent Technologies 79846-87604 Sample Loop Capillary hewlett packard HP part for HPLC



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002140731

Alltech 590 Elite Degassing System

    Price: $250.00    POD Price: $250.00    

Alltech Elite Degassing System Model 590. Single channel. 15-24v dc.


Efficient membrane constructed of highly gas-permeable PTFE AF; allows membrane volume to be reduced about two orders of magnitude less than PTFE, resulting in very quick equilibration and solvent changeover times
Low in extractables and very inert, membrane can be used with most solvents, including THF and methylene chloride ... More Info

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001140521

HP 1050 Series HPLC System

    Price: $4,599.00    POD Price: $3,500.00    

HP 1050 Series HPLC System. Includes 78855A Autosampler, 79854A Solvent Tray, 79854A Diode Array jDetector. 


Parts units

... More Info

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003140421

HP Agilent Sapphire Piston for HPLC

    Price: $99.00    POD Price: $75.00    

HP/Agilent Sapphire Piston for 1050/1100/1200 HPLC



  >>     >>  


005131213

Alltech ELSD Low Temperature Adaptor

    Price: Please Call    

Alltech ELSD Low Temperature Adaptor



  >>     >>  


006131015

Agilent Zorbax CombiHT HPLC Column XDB C8

    Price: $299.00    POD Price: $199.00    

Agilent 970100-906 HPLCColumn Zorbax

Agilent Zorbax CombiHT HPLC Column XDB-C8. 5 um, 21.2mm id x 100 mm.



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005131015

Agilent Zorbax CombiHT HPLC Column 5B C8

    Price: $301.00    POD Price: $299.00    

Agilent 870100-906 Column CombiHT

Agilent Zorbax CombiHT HPLC Column 5B-C8. 5 um, 21.2mm id x 100 mm.
... More Info

  >>     >>  


002131015

Phenomenex 03R 4253 P0 HPLC Column

    Price: Please Call    

Phenomenex, 03R-4253-P0 HPLC Column, Luna 10u C18 (2) size: 60x 21.20 mm, Particle size 10u micron.



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002130812

HP 18594B 7673 Controller for Autosampler

    Price: $450.00    POD Price: $125.00    

Agilent Hewlett Packard 18594B Autosampler interface

7673 Controller for Autosampler model 18594B.



  >>     >>  


005130404

SGE

    Price: Please Call    

SGE Syringe Cleaner, 110v. Size in inches; 6.5 l x 2 w x 2 h.



  >>     >>  


009130201

Dionex HPLC System

    Price: $11,999.00    POD Price: $10,000.00    

Dionex HPLC System. Includes, Electrochemical Detector Model ED40, Absorbance Detector AD 20, Gradient Pump GP 50, ASRS Ultra 4 mm, and PeakNet Software.



  >>     >>  


005120709

Phenomenex 00B-4043-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00B-4043-E0 HPLC Column, Luna 5u C18(2), size: 50 x 2.00 mm, Particle size 5 micron.



  >>     >>  


003120312

HP 79883-80024 4nm Optical Slit

    Price: $279.00    POD Price: $98.00    

Hewlett Packard item #79883-80024: A 4nm Optical Slit ("Spring Style") for the 1040 (II), 1050 and 1090 (II) DAD and MWD detectors



  >>     >>  


010120127

Waters Alliance Vacuum Filtration Manifold

    Price: Please Call    

Waters Alliance 289000159 Vacuum Filtration Manifold. The Alliance Filtration Manifold allows for simultaneuos filtration of up to 24 samples directly into vials housed in an Alliance sample carousel, in only seconds.

... More Info

  >>     >>  


018111222

Waters HPLC Pump Model 610

    Price: Please Call    
Waters Model 610 HPLC Pump,

  >>     >>  


004111209

Biotage Elution Solutions Flash Elute Flash System

    Price: $799.00    POD Price: $600.00    
Biotage Elution Solutions Flash Elute Flash System. Units feature a tank pressure gauge, injection selections for tank A/B, air valve selections for tank A/B, flow, vent, and various fluid line connections. ... More Info

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008111025

Alltech 340 Solvent Recycler

    Price: $399.00    POD Price: $320.00    

Alltech 340 Solvent Recyler.Desined to automatically reduce solvent consumption in isocratic HPLC applications by recycling the uncontaminated mobile phase. Usage is reduced up to 90%



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025111021

Agilent Extend-C18 LC MS Column 760450-902

    Price: $209.00    POD Price: $180.00    

Agilent 760450-902 C-18 Column

Narrow Bore ZORBAX Extend 80Å C18, 2.1 x 50 mm, 5 µm HPLC column

UNSPSC : 41115709 


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016111021

Agilent Zorbax Eclipse XDB-C18 HPLC Column

    Price: $200.00    POD Price: $199.00    

Agilent 961967-302 HPLC Column 

Agilent Zorbax Eclipse XDB-C18, 3.0 x 100 mm, 3.5 µm. The Agilent ZORBAX Eclipse XDB columns provide four bonded phase choices for method development optimization.

UNSPSC : 41115709 


... More Info

  >>     >>  


012111021

Agilent Zorbax Rx-C18 HPLC Column

    Price: Please Call    
Agilent Zorbax Rx-C18 3.5um, 4.6 x 150mm. Zorbax Rx columns are used to analyze basic compounds at low pH.

  >>     >>  


004111021

Waters Atlantis dC18 HPLC Column 186001303

    Price: $75.00    
Waters Atlantis dC18 HPLC Column 186001303. dC 18 3um, 3.0x 100mm. Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water. ... More Info

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003111021

Waters Atlantis HILIC Silica HPLC Column 186002029

    Price: $200.00    
Waters Atlantis HILIC Silica HPLC Column 186002029. 3um, 4.6 x 100mm. Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches. ... More Info

  >>     >>  


008111012

HP 79883-80028 8nm Optical Slit

    Price: $399.00    POD Price: $92.00    

Hewlett Packard item #79883-80028: A 8nm Optical Slit ("Spring Style") for the 1040 (II), 1050 and 1090 (II) DAD and MWD detectors



  >>     >>  


007111012

HP 79883-80022 2nm Optical Slit

    Price: $200.00    POD Price: $99.00    

Hewlett Packard item #79883-80022: A 2nm Optical Slit ("Spring Style") for the 1040 (II), 1050 and 1090 (II) DAD and MWD detectors



  >>     >>  


004111012

Phenomenex EV500 100 PH Synergy Column Selector

    Price: $450.00    POD Price: $450.00    
Phenomenex EV500-100-PH Synergy Column Selector.

  >>     >>  


003110920

Waters SAT-IN Module

    Price: Please Call    
Waters SAT/IN Module, 2 channel, with LAC/E connection (Cables not included)

  >>     >>  


002110717

Horizon Flash Collector

    Price: $450.00    POD Price: $450.00    
Horizon Flash Collector with Biotage Pump. Detector/Recorder, Collection Rack, Pump Module, Fash Collector, Flash compression Module, ZIF-SIM Module.

  >>     >>  


014110713

Rainin Dynamax Automatic Sample Injector Model AL-1A

    Price: $300.00    POD Price: $300.00    

Rainin Dynamax Auto Sampler Injector Model 900, 88 sample positions, Power 115/ 230 V 50/60Hz 250 W, with cooling tray 12" h x 22" l  x 12" w.



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005110513

Agilent 280959-005 Frit

    Price: $4.00    POD Price: $5.00    
Agilent Part # 280959-005. Zorbax Endcap Frit, 2um for 4.6mm id columns.

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002110513

Hewlett Packard 5062 2494 Pump Piston Seal

    Price: $199.00    
Hewlett Packard Pump Piston Seal, part number 5062-2494: 8 per pack, Piston seals for HP1090, Vespel,


  >>     >>  


010110511

Agilent 79826-87607 Autosampler Tubing

    Price: $59.00    POD Price: $40.00    

Agilent 79826-87607 Tubing

Agilent 79826-87607 Autosampler Tubing, SS Tubing, 280cm, 0.17mm id, with fittings,

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009110511

Agilent Autosampler Tubing 79826-87605

    Price: $59.00    

Agilent 79826 87605 Tubing 400mm

Agilent 79826-87605 Autosampler Tubing, SS Tubing, 400 mm, 0.12 mm id, with fittings attached

UNSPSC : 41115720 
... More Info

  >>     >>  


008110511

Agilent 79826-87602 Tubing

    Price: $89.00    

Agilent 79826-87602 Tubing

Agilent 79826-87602 Autosampler Tubing SS Tubing 280 mm, 0.12 mm inner diameter, with fittings. 

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007110511

Agilent 01090-87611 Tubing

    Price: $59.00    

Agilent 01090-87611 HPLC Tubing

Agilent 01090-87611 HPLC tubing. Capillary stainless steel 105 mm long, internal diameter 0.12 mm, non-swaged Swagelok fitting short

UNSPSC : 41115720  ... More Info

  >>     >>  


006110511

Agilent 01090-87610 Tubing

    Price: $59.00    
Agilent 01090-87610 Stainless Steel Tubing 280 mm, 0.12 mm inner diameter. 2 in package. HPLC tubing.

  >>     >>  


005110511

Agilent 01090-87610 Tubing

    Price: $59.00    
Agilent 01090-87610 Stainless Steel Tubing 280 mm, 0.12 mm inner diameter. 2 in package. HPLC tubing.

  >>     >>  


020110506

Rheodyne 7010 039 Rotor seal

    Price: $84.00    POD Price: $50.00    

Rheodyne Catalog No. 7010 039 Rotor seal for 7000, 7010, 7040



  >>     >>  


010110506

Hewlett Packard 3980 0672 Sapphire plunger

    Price: $149.00    POD Price: $100.00    

Hewlett Packard Model 3980 0672 Sapphire plunger/piston for Agilent/Hp 1090



  >>     >>  


006110506

Hewlett Packard 3980 0672 Sapphire plunger

    Price: $49.00    POD Price: $49.00    

Agilent / Hewlett Packard Model 3980 0672 Sapphire plunger/piston for Agilent/HP 1090



  >>     >>  


001110506

Hewlett Packard 5062 2465 Support Ring

    Price: $129.00    
Hewlett Packard 5062-2465 SUPPORT RING for 1050/1100

  >>     >>  


012110315

Rheodyne Model 7000 HPLC Pump Valve

    Price: Please Call    
Rheodyne Model 7000 HPLC Pump Valve

  >>     >>  


003091111

Waters Deuterium Lamp for DAD Detector

    Price: Please Call    

Waters Deuterium Lamp WAT057760 for Waters 966 2996 DAD Detector



  >>     >>  


002091110

Waters Deuterium Lamp for 484 detector

    Price: $499.00    POD Price: $400.00    

Waters Deuterium Lamp P/N 80357 for Model 484 HPLC Detector



  >>     >>  


011091109

Jasco System Controller Model 802 SC

    Price: Please Call    
Jasco System Controller Model 802 SC

  >>     >>  


014090830

Leap Technologies Auto Sampler

    Price: $9,999.00    POD Price: $6,000.00    

Leap Technologies CTC Analytics Auto Sampler, refrigerated sample compartment, with system software: Cycle Composer Version 1.5, L7, Revision 3,



  >>     >>  


004090112

HP 79853A Series 1050 Variable Wavelength UV Vis Detector

    Price: $499.00    POD Price: $399.00    

HHP Series 1050 Variable Wavelength UV/Vis Detector Model HP79853A

 



  >>     >>  


006081006

Waters Solvent Select Valve Multiport

    Price: Please Call    
Waters Solvent Select Valve Multiport

  >>     >>  


019080730

Waters HPLC Column Mode PrepPak with RCM Base

    Price: Please Call    
Waters HPLC Column Mode PrepPak with RCM Base

  >>     >>  


008080730

Hewlett Packard Model 1050 HPLC Autosampler

    Price: $699.00    POD Price: $499.00    
Hewlett Packard Model 1050 HPLC Autosampler

  >>     >>  


030080713

Pickering Laboratory Coil Assy

    Price: $499.00    POD Price: $400.00    
Pickering Laboratory Coil Assy, 0.5 ml CRX390W, 120 volts

  >>     >>  


012080603

KinteK Corporation Key Board TT6-001

    Price: Please Call    
KinteK Corporation Key Board with DC power supply

  >>     >>  


002071115

Packard Radiomatic Flo-one Beta Detector Model A-515A

    Price: Please Call    

Packard Radiomatic Flo-one Beta Radioactive Flow Detector Model A-515A



  >>     >>  


033071013

Pharmacia Biotech Deuterium Lamp Assembly Model 4090

    Price: Please Call    
Pharmacia Biotech Deuterium Lamp Assembly Model 4090, Plus a Tungsten Halogen Lamp Model 80-2106-17

  >>     >>  


005071013

Shimadzu Column Oven Model CTO-6AS

    Price: $400.00    POD Price: $400.00    

Shimadzu Column Oven Model CTO-6AS



  >>     >>  


018070916

Flux Instruments Rheos 4000

    Price: Please Call    
Flux Instruments Rheos 4000 Pump for use with Mass Spectrometry, low delay volume and extreme accuracy in delivering solvents at low flow rates.

  >>     >>  


008070516

Waters WAT080567 Assy Fan

    Price: $99.00    POD Price: $80.00    

Waters Assy Fan (system controller) M484 p/n WAT080567



  >>     >>  


X003060902

Hewlett Packard 1037A Refractive Index Detector

    Price: $550.00    POD Price: $550.00    
Hewlett Packard Refractive Index Detector, Model 1037A

  >>     >>  


012060404

Jasco 802-SC System Controller

    Price: Please Call    
Jasco Model 802-SC System Controller, Powers up with full screen.

  >>     >>  


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