HPLC Columns come in a variety of particle sizes and designs that meet different separation needs. The two important factors in choosing a column for your application are particle size and the internal diameter (ID) of the column.
The most common particle size is 5 µm silica beads. Smaller particles generally provide more surface area and better separations. However, decreasing the size of the particles while keeping the column ID size the same, while increasing the performance, will also increase the required pressure for optimum linear velocity. Larger particles are used in preparative HPLC and for non-HPLC applications such as solid-phase extraction.
The internal diameter (ID) of an HPLC Column influences the detection sensitivity and separation selectivity in gradient elution (steady changes of the mobile phase composition during the chromatographic run). Larger ID columns are usually seen in industrial applications, while small ID columns have better sensitivity and lower solvent consumption at the expense of loading capacity.
Typical manufacturers are Phenomenex, Agilent Technologies Inc., Supelco, and Waters Corporation. HiTechTrader has a multitude of HPLC Columns in various particle and ID sizes.
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Supelco Progel TSK Column G300 SW 30cm x 7.5mm. Brand new in sealed box.
Agilent Zorbax CombiHT HPLC Column XDB-C8. 5 um, 21.2mm id x 100 mm.
Monochrom HPLC Column 0504-030G212. 10u, C18
Monochrom HPLC Column 0505-030G212. 5u, C18
Phenomenex, 0GF-4252-E0 HPLC Column, Luna 5u C18 (2) size: 250 x 4.60 mm, Particle size 5 micron.
Phenomenex, 00A-4251-A0 HPLC Column, Luna 3u C18 (2) size: 30 x 1.00 mm, Particle size 3 micron.
Phenomenex 00B-4043-E0 HPLC Column, Luna 5u C5 100A, size: 50 x 4.6 mm, Particle size 5 micron.
Phenomenex, 00A-4251-E0 HPLC Column, Luna 3u C18 (2) size: 30 x 4.60 mm, Particle size 3 micron.
Phenomenex, 00A-4252-E0 HPLC Column, Luna 5u C18 (2) size: 30 x 4.60 mm, Particle size 5 micron.
Phenomenex, 00B-4255-E0 HPLC Column, Luna 5u CN 100A, size: 50 x 4.6 mm, Particle size 5 micron.
Waters Symmetry Shield RP 18 HPLC Column 186000700 is 5um and 3.0 x 100mm. These reverse-phased columns are based on Waters patented embedded polar group technology that literally shields the silica's residual surface silanols from highly basic analytes.
Phenomenex, 00G-4375-B0 HPLC Column, Synergi 4u Hydro-RP 80A size: 250 x 2.00 mm, Particle size 4 micron.
Phenomenex, 00G-3300-E0 HPLC Column, Prodigy 5 ODS (2) 125A size: 250 x 4.60 mm, Particle size 5 micron.
Phenomenex, 00F-4254-E0 HPLC Column, Luna 3u CN 100A size: 150 x 4.60 mm, Particle size 3 micron.