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HPLC Columns

Used HPLC Columns equipment for sale

HPLC Columns

HPLC Columns come in a variety of particle sizes and designs that meet different separation needs. The two important factors in choosing a column for your application are particle size and the internal diameter (ID) of the column.

Particle Size

The most common particle size is 5 µm silica beads. Smaller particles generally provide more surface area and better separations. However, decreasing the size of the particles while keeping the column ID size the same, while increasing the performance, will also increase the required pressure for optimum linear velocity. Larger particles are used in preparative HPLC and for non-HPLC applications such as solid-phase extraction.

Internal Diameter

The internal diameter (ID) of an HPLC Column influences the detection sensitivity and separation selectivity in gradient elution (steady changes of the mobile phase composition during the chromatographic run). Larger ID columns are usually seen in industrial applications, while small ID columns have better sensitivity and lower solvent consumption at the expense of loading capacity.

Top HPLC Columns Manufacturers

Typical manufacturers are Phenomenex, Agilent Technologies Inc., Supelco, and Waters Corporation. HiTechTrader has a multitude of HPLC Columns in various particle and ID sizes.

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5433 - Sepragen Corp. Superflo 5oo Column
Number of Items: 12

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003190928

Sepragen Radial Flow Chromatography Column Superflo 50

    Price: $450.00    
Sepragen Radial Flow Chromatography Column  Superflo 50,  Stainless Steel

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002190928

Sepragen Radial Flow Chromatography Column Superflo 50

    Price: $350.00    
Sepragen Radial Flow Chromatography Column Superflo 50 with column stand and smaller Superflo column

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006131015

Agilent Zorbax CombiHT HPLC Column XDB C8

    Price: $299.00    POD Price: $199.00    

Agilent 970100-906 HPLCColumn Zorbax

Agilent Zorbax CombiHT HPLC Column XDB-C8. 5 um, 21.2mm id x 100 mm.



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005131015

Agilent Zorbax CombiHT HPLC Column 5B C8

    Price: $301.00    POD Price: $299.00    

Agilent 870100-906 Column CombiHT

Agilent Zorbax CombiHT HPLC Column 5B-C8. 5 um, 21.2mm id x 100 mm.
... More Info

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002131015

Phenomenex 03R 4253 P0 HPLC Column

    Price: Please Call    

Phenomenex, 03R-4253-P0 HPLC Column, Luna 10u C18 (2) size: 60x 21.20 mm, Particle size 10u micron.



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005120709

Phenomenex 00B-4043-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00B-4043-E0 HPLC Column, Luna 5u C18(2), size: 50 x 2.00 mm, Particle size 5 micron.



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025111021

Agilent Extend-C18 LC MS Column 760450-902

    Price: $209.00    POD Price: $180.00    

Agilent 760450-902 C-18 Column

Narrow Bore ZORBAX Extend 80Å C18, 2.1 x 50 mm, 5 µm HPLC column

UNSPSC : 41115709 


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016111021

Agilent Zorbax Eclipse XDB-C18 HPLC Column

    Price: $200.00    POD Price: $199.00    

Agilent 961967-302 HPLC Column 

Agilent Zorbax Eclipse XDB-C18, 3.0 x 100 mm, 3.5 µm. The Agilent ZORBAX Eclipse XDB columns provide four bonded phase choices for method development optimization.

UNSPSC : 41115709 


... More Info

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012111021

Agilent Zorbax Rx-C18 HPLC Column

    Price: Please Call    
Agilent Zorbax Rx-C18 3.5um, 4.6 x 150mm. Zorbax Rx columns are used to analyze basic compounds at low pH.

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004111021

Waters Atlantis dC18 HPLC Column 186001303

    Price: $75.00    
Waters Atlantis dC18 HPLC Column 186001303. dC 18 3um, 3.0x 100mm. Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water. ... More Info

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003111021

Waters Atlantis HILIC Silica HPLC Column 186002029

    Price: $200.00    
Waters Atlantis HILIC Silica HPLC Column 186002029. 3um, 4.6 x 100mm. Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches. ... More Info

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019080730

Waters HPLC Column Mode PrepPak with RCM Base

    Price: Please Call    
Waters HPLC Column Mode PrepPak with RCM Base

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